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KMID : 0363020060360030613
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Journal of Korean Academy of Periodontology
2006 Volume.36 No. 3 p.613 ~ p.625
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Screening of genes differentially expressed in cultured human periodontal Ligament cells and human gingival fibroblasts
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Yoon Hye-Jeong
Choi Mi-Hye
Yeo Shin-il
Park Jin-Woo
Choi Byung-Ju
Kim Moon-Kyu
Kim Jeong-Chul
Suh Jo-Young
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Abstract
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Periodontal ligament(PDL) cells and human gingival fibroblasts(HGFs) play important roles in development, regeneration, normal function, and pathologic alteration. PDL cells and HGFs have the similarity related with general characteristics of fibroblast such as spindle shaped morphology, the presence of vimentin intermediate filament and the synthesis of interstitial collagens and fibronectin. There were many studies about the differences between PDL cells and HGFs, but they were not about whole gene level. In this study, we tried to explain the differences of gene expression profiles between PDL cells and HGFs, and the differences among three individuals by screening gene expression patterns of PDL cells and HGFs, using cDNA microarray. Although there were some variants among three experiments, a set of genes were consistentely and differentially expressed in one cell type. Among 3,063 genes, 49 genes were more highly expressed in PDL cells and 12 genes were more highly expressed in HGFs. The genes related with cell structure and motility were expressed more highly in PDL cells. These are cofilin 1, proteoglycan 1 secretory granule, collagen type I( 1), adducin gamma subunit, collagen type III( 1), fibronectin, lumican(keratan sulfate proteoglycan), and -smooth muscle actin. Tissue inhibitor of metalloproteinase known as the enzyme controlling extracellular matrix with matrix metalloproteinase is more highly expressed in PDL cells, osteoprotegerin known as osteoclastogenesis inhibitory factor is more highly expressed in HGFs. We performed northern blot to verify cDNA microarray results on selected genes such as tissue inhibitor of metalloproteinase, fibronectin, osteoprogeterin. The result of northern blot analysis showed that each cell expressed the genes in similar pattern with cDNA microarray result. This result indicates that cDNA microarray is a reliable method in screening of gene expression profiles.
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KEYWORD
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cDNA microarray, human gingival fibroblast, periodontal Ligament cell
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